A research team presents a noninvasive light-sensitive photoactivatable recombinase suitable for genetic manipulation in vivo. The highly light-sensitive property of photoactivatable Flp recombinase ...

We have previously shown that a dual system for controlling gene expression that relies both on transcriptional regulation and DNA recombination mediated by the site-directed recombinase, Flp, ...

A new molecule, photoactive Flp recombinase, has been developed for use in an optogenetic technique that can deliver non-invasive genetic manipulation in the deep brain tissue of mice. A research team ...

Insulators define independent genomic regions that are protected from the influence of distant regulatory sequences. In the 1 November EMBO Journal, Parnell and Geyer describe a novel application of ...

Anyone who's ever made stable cell lines knows that clones can vary wildly in terms of expression levels. Some express the transfected gene at high levels, some express at low levels, and some, for ...

In traditional knockout studies, the gene is deleted altogether. However, deleting genes that are essential to embryonic development is lethal. Conditional gene knockout can be activated in an adult ...

Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, No. 2 (January 11, 2011), pp. 498-503 (6 pages) Routine manipulation of cellular genomes is contingent upon ...