DNA Helicase | Overview, Definition & Function
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Helicase in DNA Replication | Definition & Function
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DNA gyrase, often referred to simply as gyrase, is an enzyme that relieves strain while double-strand DNA is being unwound by helicase. It is also known as DNA topoisomerase II. This causes negative supercoiling of the DNA. Bacterial DNA gyrase is the target of many antibiotics, including nalidixic acid, novobiocin, and ciprofloxacin. It supercoils (or relaxes positive supercoils) into DNA by looping the template so as to form a crossing, then cutting one of the double helices and passing the ot
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The replisome complex in action: During DNA replication, helicase unzips the parent strands while single stranded binding proteins stabilize each strand. Topoisomerase relieves tension by cutting and rejoining DNA downstream of the replication fork. The primase enzyme catalyzes an RNA primer so that DNA polymerase III (guided by a sliding clamp) can begin polymerizing the new DNA molecule from the template. On the leading strand, this happens continuously as helicase continues unzipping the pare
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